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KMID : 1134820230520020154
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Journal of the Korean Society of Food Science and Nutrition
2023 Volume.52 No. 2 p.154 ~ p.161
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Comparative Analysis of Bioactive Compounds in Artemisia capillaris Thunberg and Artemisia iwayomogi Kitamura and Validation of an Analytical Method for Scoparone in Artemisia capillaris
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Jung Sang-Mi
Lee Yong-Jun
Ra Moon-Jin
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Abstract
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Artemisia belongs to the daisy family Asteraceae, which includes species like mugwort, wormwood, and sagebrush. Of the Artemisia genera most used in Korea, we noted that Artemisia capillaris Thunb. and Artemisia iwayomogi Kitamura, were being used interchangeably. This study reveals differences between the components of A. capillaris Thunb. and A. iwayomogi Kitamura. Using an HPLC method developed by our research team, 10 candidate bioactive compounds were identified in the 100% ethanol and distilled water extracts of A. capillaris Thunb. and A. iwayomogi Kitamura. Chlorogenic acid and scoparone were the most abundant components in A. capillaris Thunb. but were not detected in A. iwayomogi Kitamura. The devised method was validated for the determination of scoparone in A. capillaris extracts according to the ICH guideline with respect to specificity, precision, accuracy, and linearity. Calibration curves showed good linearity (R2>0.9999), and inter- and intra-day precisions of scoparone in A. capillaris were 6.79% and 2.00∼3.88%, respectively. Recoveries of quantified compounds ranged from 100.21 to 106.37%. Thus, the devised method was validated for scoparone in A. capillaris. Our results suggest that the method could provide basic data to aid the development of therapeutic agents or the commercialization of health functional foods.
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KEYWORD
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Artemisia capillaris, scoparone, marker compound, HPLC, analytical method
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